Recirculating Chillers
Sigma Aldrich Fine Chemicals Biosciences TranscreenerR ADP2 fI Assa10k
The Transcreener(R) HTS Assay platform overcomes the need for time-consuming one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener(R) HTS Assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. The Transcreener(R) ADP2 fluorescent intensity (fI) assay extends the Transcreener(R) platform for ADP detection by utilizing a simple fluorescent intensity output which can be used on both fluorescence readers typically found in academic and therapeutic research labs as well as more complex multimode plate readers more commonly used in core facilities and HTS labs. The Transcreener(R) ADP2 fI Assay is a red competitive fluorescence intensity (fI) assay based on the detection of ADP and therefore is compatible with any enzyme class
Sigma Aldrich Fine Chemicals Biosciences fastStartTM High fidelity
The fastStart(TM) High fidelity PCR System is a blend of fastStart Taq DNA Polymerase and a thermostable proofreading protein which is also chemically modified. This protein mediates proofreading activity but carries no polymerase activity. Both proteins are inactive below 75 C and are activated by heating to 95 C for two minutes.Hot start protocols have improved specificity sensitivity and PCR yield. Heat-labile blocking groups on amino acid residues of fastStart(TM) Taq DNA Polymerase make the enzyme inactive at room temperature. There is no elongation at low temperatures when primers bind nonspecifically. fastStart(TM) Taq DNA Polymerase is activated by removal of blocking groups using a preincubation step at 95C. The fastStart(TM) High fidelity PCR System is the product of choice for multiplex PCR. fastStart(TM) High fidelity Enzyme Blend (5 U/mul) in storage bufferfastStart(TM) High fidelity Reaction Buffer 10x concentrated with 18 mM MgCl2fastStart(TM) High
Sigma Aldrich Fine Chemicals Biosciences Pyrethrum extract Unted S100MG
This product is provided as delivered and specified by the issuing Pharmacopoeia. All information provided in support of this product including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia.for further information and support please go to the website of the issuing Pharmacopoeia.
Sigma Aldrich Fine Chemicals Biosciences TRI ReagentR for processin
TRI reagent(R) is a quick and convenient ready-to-use reagent useful for efficient total RNA extraction or for the simultaneous isolation of RNA DNA and protein. TRI reagent(R) is a mixture of guanidine thiocyanate and phenol in a monophasic SOLUTIONon. Homogenization or lysis of the tissue sample in TRI reagent(R) dissolves RNA DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases an aqueous phase containing the RNA the interphase containing DNA and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.
Sigma Aldrich Fine Chemicals Biosciences TrizmaR hydrochloride solu1L
A series of Pre-mixed SOLUTIONons of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy /- 0.1 pH units.
Sigma Aldrich Fine Chemicals Biosciences AldrichR KugelrohrTM short1EA
Quickly Distills Difficult Materials with Minimal Hold-up and LossHigh Vacuum Capability and Safe Front Loading DesignDistillation Applications.nbspHeat-sensitive compounds.nbspLiquids and low-melting solids from polymers and tars.nbspRemoves color and particulates.nbspSuitable for sublimation and solvent evaporationFeatures.nbspAir bath oven distills up to 230C.nbsp25 mL to 2 L flask capacity.nbspFront Loading oven door with safety glass viewing window.nbspInterchangeable PTFE support bearings for flask joint 14/20.nbspDigital thermocouple temperature control.nbspFront panel with controls for temperature vacuum and motor drive.nbspAdjustable receiving flask cooling bath.nbspOscillating drive motorSpecifications.nbspOven flask size range 25 mL to 2 L.nbspHeating range ambient to 230C /- 3C across span after stabilization.nbspPower 120/240 VAC /- 10% 50/60 Hz /- 3% detachable power cord.nbspFuses Heater 7.5 A Vac 2.5 A Power input 10 A.nbspHeater 900 W.nbspSize 25 in. W x 11 in.
Sigma Aldrich Fine Chemicals Biosciences RNAzolR RT for processing
RNAzol RT is a quick and convenient reagent for use in thesingle-step isolation of total andsmall RNA from biological samples of human animal plant yeast bacterial and viral origin. A convenient single-step liquid phase separation results in the isolation of RNA from DNA protein polysaccharides and other molecules. RNAzol(R) RT can be used to isolate separate fractions of mRNA and micro RNA or to isolate total RNA containing all classes of RNA in a single fraction. This product a mixture of guanidine thiocyanate and phenol in a monophase SOLUTIONon effectively dissolves DNA RNA and protein on homogenization or lysis of tissue sample. Addition of water to the mixture allows for the precipitation of DNA proteins polysaccharides and other molecules which can then be removed by centrifugation. RNA can then be isolated by alcohol precipitation washing and solubilization. Chloroform-induced phase separation is not necessary. One mL of RNAzol(R) RT is sufficient to isolate RN
Sigma Aldrich Fine Chemicals Biosciences CelLytcTM B Cell Lyss Re500ML
CelLytcTM B Cell Lyss Re500ML
Sigma Aldrich Fine Chemicals Biosciences Cardiolipin solution from100MG
Cardiolipin SOLUTIONon from100MG
Sigma Aldrich Fine Chemicals Biosciences Oversulfated Chondroitin S10MG
This product is provided as delivered and specified by the issuing Pharmacopoeia. All information provided in support of this product including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia.for further information and support please go to the website of the issuing Pharmacopoeia.
Sigma Aldrich Fine Chemicals Biosciences Deoxyribonucleic acid from100G
Deoxyribonucleic acid from100G
Sigma Aldrich Fine Chemicals Biosciences JumpStartTM Taq ReadyMixTM
JumpStart(TM) Taq ReadyMix(TM) is a ready-to-use 2X master mix that contains JumpStart(TM) Taq DNA polymerase 99% pure dNTPs reaction buffer and JumpStart(TM) Taq antibody. The Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70C the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.
Sigma Aldrich Fine Chemicals Biosciences Caprylocaproyl polyoxylgy200mg
This product is provided as delivered and specified by the issuing Pharmacopoeia. All information provided in support of this product including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia.for further information and support please go to the website of the issuing Pharmacopoeia.